2010年上海市食源性沙门菌菌型分布和药敏分析以及快速检测方法的建立

Foodborne Salmonella Serotype Distribution and Sensitivity Analysis in Shanghai in 2010

目的:通过对2010年上海市市售食品中食源性沙门菌污染状况、菌型分布及药敏试验进行分析,初步确定该地区食源性沙门菌的菌型分布及耐药性,为防治因沙门菌感染引起的食源性疾病提供科学依据,并摸索建立了针对沙门菌的快速检测方法。方法:对农贸市场和超市的5类840份市售食品进行沙门菌分离鉴定及耐药性分析,并根据沙门菌的侵袭蛋白A(invA)基因序列设计保守引物,特异性快速检测沙门菌。结果:本次市售食品沙门菌阳性率检出率为4.29%,共36株沙门菌,生禽畜肉所占比例高达91%。主要血型为鼠伤寒沙门菌,德尔卑沙门菌和都柏林沙门菌。36株沙门菌药敏分析显示:所有菌株对头孢类等抗生素敏感较高,但对氨苄西林、麦迪霉素、环丙沙辛、呋喃妥因等存在普遍较多耐药菌株,并在此基础上通过PCR法成功地特异性检测出沙门菌。结论:上海市市售食品沙门菌污染以生禽畜肉为主,对抗菌药物的耐药性较高。目前治疗市售食品中食源性沙门菌引起的食源性疾病应首选头孢内等抗生素。另外PCR快速检测方法也操作简单,特异性强,灵敏度高,对食品中沙门菌污染能起到快速检测和监控。

Objective： To provide scientific basis for controlling diseases caused by saimonellas through understanding their pollution states, distribution of bacterial types and drug resistances of market foods in Shang Hai, and we establishrapid detection method for Salmonella. Methods： 840 food Salmonella isolation identification and drug resistance analysis from The farmers market and supermarket, the primers according to the Salmonella typhi invasion protein A（invA）was designed and was PCR to detect the Salmonella. Results： In all food relevance ratio of saimonellas is 4.29%,the highest is 91% in raw livestock meat.Main serotype Salmonella typhimurium is salmonella typhimurium, del weakpulse, duplin.The strains is antibiotics sensitive to ceflazidime,but is resistance to ampicillin,midecamycin, ciprofloxacin, nitrofurantoin,And on this basis through PCR method successfully specificity detection of Salmonella. Conclution： saimonellas pollution of market foods in Shanghai is predominated covered by raw livestock meat.Current treatment of market food in foodborne Salmonella foodbome disease should be the first choice of the antibiotic, and PCR rapid detection method also has the advantages of simple operation, strong specificity, high sensitivity of Salmonella contamination in food can play a quick detection and monitoring.