LDH-C:睾丸重要的特异表达基因

LDHC:The most important testis specific gene

乳酸脱氢酶C(LDHC)是目前已知的最早在男性生精细胞中发现的睾丸特异同功酶。LDHC最早通过凝胶电泳技术在人精子及睾丸生精细胞中被发现。免疫组化结果显示LDHC最早出现在早期的粗线期初级精母细胞中,其数量随着减数分裂逐渐增加。成熟精子中LDHC主要定位在精子尾部的主段区域。研究显示,乳酸脱氢酶家族的同功酶在哺乳动物细胞中无所不在,他们受到发育的调控,具有组织细胞的特异性,且功能多样。本文就LDHC的发展史及他们在帮助精子完成受精过程中的作用作一综述。

Lactate dehydrogenase （LDHC） was the first testis specific isozyme discovered in male germ cells. LDHC was detected initially in human spermatozoa and spermatogenic cells of the testes by gel electrophoresis, lmmunohistochemistry showed that LDHC was first existed in early pachytene primary spermatocytes with an apparent increase in quantity following meiosis, and it located in and on the principle piece of the sperm tail. Studies showed that the lactate dehydrogenase isozymes are ubiquitous in vertebrates, developmentally regulated, tissue and cell specific and multi-functional. Here we review the history of LDHC and the function of LDHC required for sperm to accomplish their ultimate goal, fertilization.