热休克蛋白60与心房颤动的关系

The relationship between heat shock protein-60 and atrial fibrillation

目的探索心房颤动(简称房颤)与氧化应激的关系。方法以住院行心脏换瓣手术的病人作为研究对象,按患者心律分为房颤组(n=22)和对照组(n=20),对照组为窦性心律。所有研究对象的心房肌组织样本,均作病理形态学观察,并用免疫组织化学法检测其热休克蛋白60(HSP60)表达;用乳胶增强免疫比浊法测定其血清C-反应蛋白(CRP)水平。结果房颤病人的心肌细胞肥大与间质纤维化程度都比对照组明显。HSP60表达阳性率房颤组高于对照组(68.2%vs 25.0%),灰度值房颤组低于对照组(157.69±16.00 vs 178.69±28.63)。血清CRP含量房颤组高于对照组(20.72±17.92 mg/L vs 6.80±6.26 mg/L,P均<0.05)。结论氧化应激与房颤关系密切。

Objective To investigate the relationship between atrial fibrillation （AF） and oxidative stress. Methods Forty-two of in-patients who were operated for valve replacement served as the subjects of this study;The AF group was composed of 22 AF patients and the control group was composed of 20 patients with sinus rhythm. Atrial muscle samples of all subjects were examined pathologically for morphology characteristics. Serum heat-shock-protein 60 （HSP60） expression levels were detected by means of immuno-histo-chemistry, and C-reactive protein （CRP） levels were detected as well by means of latex-enhanced immuno-turbidimetry. Results Degree of hypertrophy of the atrial myocytes and pathyfibrosis looked more obviously in AF group, than that in control group. The positive rate of serum HSP-60 expression in AF group was significantly higher than that in control group;the gray degree of HSP-60 in AF group was significantly lower than that in control group. The mean level of serum CRP in AF group was significantly higher than that in control group（all P 〈0. 05 ）. Conclusion Oxidative stress might be closely related to atrial fibrillation.