4,5-二溴苯基荧光酮—CTMAB荧光熄灭法测定微量铜的研究

Study on the Fluoro-Quenching Method for the Determination of Trace Copper with 4, 5-Dibromophenyl Fluorone in the Presence of CTMAB

本文报道了在CTMAB存在下,以4,5-二溴苯基荧光酮(DBPF)作为荧光试剂,用荧光熄灭法测定微量铜的新方法。详细研究了各种条件实验,反应的适宜酸度范围为pH=6.4～8.0,铜量在0.1～5.0μg/25ml范围内呈线性关系,配合物的组成比为Cu∶DBPF=1∶2,激发波长为362nm,发射波长为560nm。本法的灵敏度高,已用于测定金属镍和金属钴中的微量铜,获得了颇为满意的结果。

A new method for fluoro-quenching determination of trace Cu(II) with DBPF and CTMAB has been described. The optimum condition is studied. Rectilinear range is 0,1-5,0ug/25ml at pH6.4-8.0. The molar ratio of Cu to DBPF is 1 : 2. The excitation and emission wavelengths are 362 nm and 560 nm, respectively. The method proposed has been successfully applied to the direct determination of trace Cu in pure cobalt and pure nickel.