HPLC法同时测定阿托伐他汀钙片与普罗布考片中2主分含量

Simultaneous Content Determination of Atorvastatin Calcium Tablet and Probucol Tablet by HPLC

目的:建立同时测定阿托伐他汀钙片与普罗布考片中2主分含量的方法。方法:采用高效液相色谱法。色谱柱为Di-阿am托on伐sil他C汀18;钙流和动普相罗为布甲考醇检-醋测酸浓铵度缓线冲性液范(围pH分4别.0)为=59.50～∶55,0流.0(速r为=01..909m9L9.)、m1i0n.-01～;检10测0.波0μ长g.为mL24-(61nr=m;0选.99用9丹9)皮,平酚均为加内样标回。收结率果分:别为100.5%(RSD=1.05%,n=9)、99.6%(RSD=0.63%,n=9)。结论:此法简便、准确、重复性好,可以为同时测定二者在制剂中的含量或受试体中的血药浓度提供方法依据。

OBJECTIVE：To establish a method for simultaneous determination of the concentration of atorvastatin calcium and probucol. METHODS： HPLC method was adopted. A Diamonsil C18 column was used with the mobile phase consisted of methanol-ammonium acetate buffer solution （pH 4.0） （95 ： 5） at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 246 nm. The internal standard was paeonolum. RESULTS： The linear ranges of atorvastatin calcium and probucol were 5.0- 50.0 μg·mL^-1（r=0.999 9） and 10.0-100.0 μg·mL^-1, respectively （r=0.999 9）. The recovery of atorvastatin calcium was 100.5% （RSD： 1.05%, n=9） and that of probucol was 99.6% （RSD=0.63%, n=9）. CONCLUSION： This method is simple, accurate and reproducible, which can be provided for eveidence for the content determination of atorvastatin calcium and probucol and their blood concentration.