液相色谱-质谱联用法同时测定生脉注射液中人参皂苷Rg_1、Re、Rb_1和五味子醇甲在健康人血浆的浓度

Determination the concentration of ginsenoside Rg_1,Re,Rb_1 and schisantherin A of shengmai injection in healthy human plasma by LC-MS/MS

目的建立液相色谱-质谱联用法测定生脉注射液(心血管系统药)中人参皂苷Rg1、Re、Rb1和五味子醇甲在健康人血浆的浓度。方法血浆样品用乙腈沉淀法处理,用电喷雾离子化和正离子多离子反应监测方法,检测人参皂苷Rg1、Re、Rb1和五味子醇甲。结果 Rg1、Re、Rb1和五味子醇甲的线性范围分别为0.64～509.000,.62～501.00,1.180～4725.00,0.10～153.00μg.L-1;定量下线分别为0.64,0.62,1.18,0.10μg.L-1;方法回收率分别为78.55%～91.65%7,8.57%～104.98%,79.62%～93.17%,82.50%～104.80%;日内、日间RSD值均小于15%。结论该法准确、灵敏、特异,适用于血浆中生脉注射液中4种成分浓度的同时测定。

Objective To develop a LC-MS/MS method for the determination the concentration of ginsenoside Rg1,Re,Rb1 and schisantherin A of shengmai injection in healthy human plasma.Methods Plasma samples were pretreated by acetonitrile,ginsenoside Rg1,Re,Rb1 and schisantherin A was detected by the positive electrospray ionization method under multiple reaction monitoring mode.Results The calibration curve of ginsenoside Rg1,Re,Rb1 and schisantherin A in plasma was linear over the range of 0.64-509.00,0.62-501.00,1.18-4725.00 and 0.10-153.00 μg·L-1.The limit of quantitation was 0.64,0.62,1.18 and 0.10 μg·L-1.The relative were 78.55%-91.65%,78.57%-104.98%,79.62%-93.17% and 82.50%-104.80%,respectively.The within-day and between-day RSDs were less than 15%.Conclusion This method is accurate,sensitive,and specific,it is suitable for the measurement of plasma ginsenoside Rg1,Re,Rb1 and schisantherin A concentration.