小儿实体肿瘤切除术前后血清皮质醇和IL-6水平变化研究

Research of changes of serum levels of cortisol and IL-6 in children pre and post operations

目的探讨婴幼儿手术前后血清皮质醇和IL-6水平的变化规律,分析两者在手术应激时的意义。方法取14例实体肿瘤择期手术患儿(3～6岁)术前24 h、术后4 h、术后24 h、术后48 h及术后72 h的血清,用免疫组织化学法和ELISA法检测皮质醇和IL-6表达水平。结果血清皮质醇和IL-6分别在术后24 h和4 h开始升高,并在术后72 h内降至术前水平。结论血清中皮质醇和IL-6的变化能反映人体对手术应激的耐受程度,可能作为推测实体肿瘤患儿术后恢复情况的敏感指标。

【Objective】 To observe the variation and regularity of cortisol and IL-6 in children blood serum pre and post operations,then analyze the role of these indexes in the surgical stress.【Methods】 The time to draw blood were at 24 hours pre-operation,4 hours,24 hours,48 hours,and 72 hours post-operation,then The expression of cortisol was detected by immunohistochemical method,and IL-6 by ELISA.【Results】 The blood serum of cortisol and IL-6 rise 24 hours post-operation and 4 hours post-operation,then decreased to pre-operation levels 72 hours post-operation.【Conclusions】 The change of cortisol and IL-6 in blood serum can reflex the ability of tolerance to operation,would be sensitive markers to presume and judge children＇s condition after operations.