摘要
目的研究银杏内酯K对脑缺血的保护作用。方法采用小鼠常压耐缺氧实验,测定小鼠存活时间;小鼠双侧颈总动脉结扎引起急性不完全脑缺血模型,测定小鼠喘气时间、脑乳酸含量和碱性磷酸酶活性,观察组织形态学、脑指数和脑含水量的变化。结果银杏内酯K能剂量依赖性地延长小鼠缺氧缺血的存活时间;延长喘气时间,减少乳酸含量,降低碱性磷酸酶活性,减小脑指数和脑含水量。病理学组织检查显示,银杏内酯K能改善脑缺血再灌注造成的小鼠神经细胞的损伤,减少组织坏死。结论银杏内酯K对脑缺血有保护作用。
OBJECTIVE To study the protective effect of ginkgolide K on cerebral ischemia.METHODS Anoxia was produced by close normobaric hypoxia;the survival time of mice was determined;acute incomplete cerebral ischemia was induced by ligaturing bilateral carotid arterise;breath time,brain lactic acid content and alkaline phosphatase activities,brain index and brain water content were detected,morphologic change was observed.RESULTS Ginkgolide K prolonged the survival time of hypoxic-ischemic mice in dose-dependent manner.In acute incomplete cerebral ischemia mice,ginkgolide K extended breathing time,reduced lactic acid content,decreased alkaline phosphatase activity,lessened brain index and brain water content.Moreover,pathological examination showed that ginkgolide K ameliorated the cerebral ischemia-reperfusion injury in mice nerve cells and reduced tissue necrosis.CONCLUSION Ginkgolide K has a protective effect on ischemia brain damage.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第10期877-880,共4页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金(81001384)
广州市科技计划项目(2010GK-C041)
关键词
银杏内酯K
脑缺血
缺氧
乳酸
碱性磷酸酶
脑含水量
ginkgolide K
cerebral anoxia
cerebral ischemia
lactic acid
alkaline phosphatase
brain water content
