槐花总黄酮对大鼠糖尿病模型血清胰岛素、瘦素和C-肽水平的影响

Effects of Total Flavonoids of Sophorae Flos on the Levels of Insulin,Leptin and C-Peptide in Serum in Diabete Rat Model

目的探讨槐花总黄酮对链脲佐菌素性大鼠糖尿病模型血清胰岛素、瘦素和C-肽水平的影响。方法以舌下静脉注射链脲佐菌素60 mg.kg-1制备大鼠糖尿病模型,随机分为5组:槐花总黄酮高(600 mg.kg 1)、中(300 mg.kg 1)、低(150 mg.kg 1)剂量组、二甲双胍组(208 mg.kg 1)和模型组(给予同体积生理盐水)。另取健康大鼠设为空白对照组,给予同体积生理盐水。每天给药1次,连续给药30 d,于第10,20,30天分别测空腹血糖值,第30天测血清胰岛素、胰岛素抗体、瘦素和C-肽水平。结果模型组从实验开始至第30天血糖水平显著升高,第30天血清瘦素水平显著升高,血清胰岛素水平及C-肽水平显著降低,说明链脲佐菌素大鼠糖尿病模型制备成功。与模型组比,槐花总黄酮各剂量组第20,30天可显著降低模型大鼠血糖水平,第30天可显著升高血清胰岛素水平和C-肽水平,显著降低血清瘦素水平。结论槐花总黄酮对链脲佐菌素性大鼠糖尿病模型有良好的治疗作用,可使血清葡萄糖及瘦素水平显著降低,血清胰岛素水平和C-肽水平显著升高。

OBJECTIVE To investigate the effect of total flavonoids of Sophorae Flos（TFSF） on the levels of insulin in serum,leptin and C-peptide in diabete rats which was established by streptozotocin.METHODS The diabete rat model was established by injected with 60 mg·kg-1 streptozotocin,and then randomized into 5 groups： large dosage TFSF group（600 mg·kg-1）,middle dosage TFSF group（300 mg·kg-1）,small dosage TFSF group（150 mg·kg-1）,metformin group（208 mg·kg-1）,and model group.Control group was set up with healthy normal rats.All the rats were administrated once a day for 30 days.Blood glucose in the limosis were measured on the 20,30 day and then rats were sacrificed.The levels of insulin,insulin antibody,leptin,C-peptide in orbital blood were measured.RESULTS Compared with control group,the level of blood sugar and leptin in model group increased obviously,the level of insulin and C-peptide decreased obviously.It illustrated that the diabete model was established successfully.Compared with model group,the level of blood sugar in TFSF groups obviously decreased after 20 or 30 days,the level of insulin and C-peptide increased obviously after 30 days,while the level of leptin decreased obviously.CONCLUSION TFSF has good hypoglycemic effect on the diabete rats established by injection of streptozotocin.It can decrease the level of blood sugar and leptin,and increase the level of insulin and C-peptide significantly.