摘要
[目的]诱变和筛选高产纤维素酶的绿色木霉菌种。[方法]利用紫外线对出发菌株进行诱变,经过初筛和发酵检测挑选出高产纤维素酶的菌株。[结果]筛选到高产纤维素酶的绿色木霉K6,酶活是出发菌株的1.39倍。[结论]诱变筛选得到的K6菌株高产纤维素酶,为秸秆纤维素的利用奠定了基础。
[Objective] The aim of this study was to find out a new Trichoderma vride K strain highly producing cellulase.[Method] Ultraviolet（UV） was used to induce mutagenesis on T.vride K and to select out a new Trichoderma vride strain highly producing cellulase from the first round and further selection.[Result] A new T.vride strain K6 with high yield of cellulase was obtained with the enzyme production amount of 1.39 times over that of starting strain K.This strain showed highest cellulase yield under the culture condition of 28 ℃ for 96 h.[Conclusion] The strain K6 selected out from induced mutation is endowed with better capacity of producing cellulase,which provides a new method for the utilization of straw.
关键词
绿色木霉
纤维素酶
诱变
Trichoderma vride
Cellulase
Mutagenesis
