叶酸的提取及抗氧化性研究

Study on extraction conditions and antioxygenic activity of folic acid

研究果蔬中叶酸的最佳提取条件及叶酸的抗氧化活性。以油菜,香菇,香蕉为原料,以提取温度、提取剂的浓度、提取时间为因素进行正交试验,确定最佳提取条件,用荧光光度法测定叶酸含量。采用Fenton体系、邻苯三酚自氧化体系,分别研究不同浓度的叶酸对羟自由基(.OH)和超氧自由基(O2-.)的清除效果。结果表明果蔬中叶酸的最佳提取条件为:提取温度100℃、提取时间45 min、提取剂浓度40%。在所选浓度范围内叶酸对羟基自由基、超氧阴离子均有较强的清除效果,且清除效果与叶酸浓度呈一定的量效关系,相同浓度的叶酸对羟基自由基和超氧阴离子自由基的清除能力有所不同,叶酸浓度在0.062 mg/mL时对羟基自由基的清除率为90.73%,而此时对超氧阴离子自由基的清除率为66.54%,而且随着反应时间的延长,清除率下降。结论为不同果蔬中叶酸含量有较大差异,叶酸对羟自由基和超氧阴离子都有较强的清除作用。

This paper mainly studied the best extraction conditions and antioxidant activities of folic acid. Fluorescence detection method of lttminescencethe is used to determine the content of folic acid. To determine the optimum determination conditions by orthogonal test, the scavenging effects of different concentration of folic acid on hydroxyl free radical （OH） and superoxide anion free radicals （O2） by Fenton reaction and pyrogallic autoxidation. The results showed that the optimum conditions were extraction temperature 100 ℃, extraction time 45rain, and the concentration of extraction 40%. Folic acid has good scavenging effects on ＂OH and O2＂. Within the selected concentration, the scavenging effects on hydroxyl free radicals had certain dose dependent relationship with folic acid concentration. When the concentration of folic acid reached 0.062mg/mL, the maximum scavenging rate on OH is 90.73% and the maximum scavenging rate on O2 was 66.54 %. As prolonged, the scavenging rate goes down. The content of folic acid in different garden stuff has obvious difference. Folic acid has preferable scavenging effect on hydroxyl free radical （OH） and superoxide anion flee radicals （02）.