灯盏花素对抗结核药致小鼠肝损伤的影响

Effect of Breviscapine on hepatic injury induced by anti-tuberculosis drugs in mice

目的:观察灯盏花素对抗结核药致小鼠肝损伤的影响。方法:采用异烟肼和利福平制造小鼠肝损伤模型,将40只小鼠随机分为对照组、模型组、护肝片组、灯盏花素低剂量组和高剂量组,每组各8只,分别灌胃,每日1次,持续两周。取血,检测谷草转氨酶(AST)水平;取肝,观察肝病理学变化。结果:灯盏花素高剂量组和护肝片组均能明显降低肝损伤小鼠血清AST水平(P<0.05、P<0.01);病理学检查显示,与对照组比较,模型组肝体积增大,颜色变浅,镜下出现严重的肝细胞变性、坏死及炎细胞浸润;与模型组比较,灯盏花素低剂量组肝体积缩小,肝细胞损伤明显减轻;与灯盏花素低剂量组比较,灯盏花素高剂量组肝细胞轻度变性。结论:灯盏花素对抗结核药致小鼠肝损伤具有保护作用。

Objective： To observe the effect of Breviscapine on hepatic injury in mice induced by anti-tuberculosis drugs. Methods： Isoniazid and Rifampicin were used in mice as model of hepatic injury. 40 mice were randomly divided into control group, model group, hugan tables group, low and high dose of Breviscapine groups, each group had 8 rats. Gastric perfusion was administered once a day for two weeks. The level of aspartate transaminase （AST） in serum and the pathology changes of liver were detected. Results： The level of AST in serum for mice with hepatic injury significantly decreased both in high dose of Breviscapine group and hugan tables group （P〈0.05, P〈0.01）; compared with control group, pathology examination showed liver magnify and colour shoal, degeneration and necrosis of hepatic cells and inflammatory cellular infiltration were observed in the model group, compared with model group, hepatic bulk and hepatic injury were evidently alleviated in low dose of Breviscapine group; compared with low dose of Breviscapine group, liver cell mild denaturalization in high Breviscapine group. Conclusion： Breviscapine has a protective effect on hepatic injury induced by anti-tuberculosis drugs in mice.