炮制对巫山淫羊藿黄酮类成分的影响

Effect of processing on total flavonoids and flavonoid monomer in Epimedium wushanense T.S.Ying

目的:为巫山淫羊藿饮片质量控制指标的选择提供科学依据。方法:对贵州不同产地的巫山淫羊藿生品和油炙品饮片,采用紫外-可见分光光度法测定总黄酮含量,HPLC测定朝藿定C和淫羊藿苷含量。结果:巫山淫羊藿炮制后总黄酮含量降低,淫羊藿苷含量升高,朝藿定C含量有升有降。结论:巫山淫羊藿饮片以总黄酮、朝藿定C和淫羊藿苷等多指标进行质量评价为佳。

Objective：To provide the scientific evidence on the selection of quality control indicators in Epimedium wushanense T.S.Ying.Methods：The contents of total flavonoids and flavonoids monomer composition in raw product and oil broiled product were determine.UV-spectrophotometry was used to determine the contents of total flavonoids,HPLC was used to determine the content of Epimedin C and Icariin.Results：The contents of total flavonoids were lower,Icariin were higher,Epimedin C were higher or lower after processing.Conclusion：The best method to evaluate the quality of Epimedium wushanense T.S.Ying.is the total flavonoids,Epimedin C,Icariin and other indicators.