PCR-DGGE研究Carrousel 2000氧化沟工艺调试过程中的微生物多样性

Application of PCR-DGGE to Study Microbial Diversity During the Operating of Carrousel 2000 Oxidation Ditch

收集Carrousel 2000型氧化沟工艺启动过程中活性污泥样品,直接提取微生物的基因组DNA并纯化,然后对细菌16S rDNA的V3高变区进行PCR扩增和DGGE分离,通过比较DGGE图谱的相似性来研究工艺调试过程中微生物种群的变化情况.研究表明,活性污泥中具有非常丰富的微生物种群.调试初期水质波动对氧化沟中微生物种群的影响非常明显,但接种的成熟活性污泥中微生物种群能够很快适应新型氧化沟工艺的结构及水力特性.调试从4月开始,4月氧化沟中微生物种群相似性Cs最大值为68.9%,5~6月Cs最大值为70.8%,8月Cs最大值为73.0%,可见氧化沟中微生物种群相似性逐渐增加,直至稳定.在此过程中,系统对COD、氨氮的处理效果同步提高并趋于稳定.综合分析好氧活性污泥在氧化沟中驯化期为2个月.

To reveal microbial diversity of aerobic activated sludge in Carrousel 2000 oxidation ditch,the sludge in the startup of the oxidation ditch was sampled and their genomic DNA was extracted directly and purified,then the 16S rDNA genes（V3 region） were amplified,and denaturing gradient gel electrophoresis（DGGE） was used to detect microbial diversity of the sludge in oxidation ditch starting-up.The DGGE profiles revealed that microbial population in the activated sludge was very rich.Wastewater quality variation at initial stage had obvious effects on the microbial population in oxidation ditch till two months later.But the microbial population in inoculated activated sludge could adapt quickly to structure and hydraulic properties of the new oxidation ditch process.The operation started from April,and the maximal similarity coefficient of the microbial population in oxidation ditch was 68.9% in April,70.8% from May to June,and 73.0% in August,respectively.This showed that the similarity coefficient of the microbial population increased gradually and remained stable.During this process,the removal effectiveness of COD and ammonia nitrogen also increased gradually and tended to stable.The comprehensive analysis indicated that it was taken about two months for domesticating the aerobic activated sludge in Carrousel 2000 oxidation ditch.Fig 4,Tab 4,Ref 12