核桃外植体的组织培养

Tissue Culture of Walnut Explant

[目的]研究核桃组织培养与快速繁殖的方法。[方法]以"金薄香"1号带腋芽的茎段为外植体进行试管繁殖。[结果]腋芽萌生最佳培养基:DKW+BA1.5mg/L;分化及继代最佳培养基:DKW+BA0.4mg/L+IBA0.01mg/L;生根最佳培养基:1/2DKW+IBA1.0mg/L,不过生根率只有23.3%,效果不太理想;二步生根法效果不错,生根率可达到36.7%。[结论]该研究筛选获得适宜"金薄香"1号核桃快繁的最佳培养条件,为建立核桃快繁体系、扩大核桃苗繁育规模奠定基础。

[Objective] The aim was to study the method of tissue culture and rapid propagation of walnut.[Method] The explants were collected from stem with axillary buds,the tube propagation of Jinboxiang1 was studied.[Result] The optimum culture medium for germination of the axillary buds：DKW＋BA 1.5 mg/L;for differentiation and subculture：DKW＋BA 0.4 mg/L＋IBA 0.01 mg/L;for rooting：1/2 DKW＋IBA 1.0 mg/L,however,rooting efficiency was only 23.3%,this result was not satisfied.The method of taking root with two steps was ideal,and taking root efficiency reached to 36.7%.[Conclusion] The optimal culture conditions of rapid propagation of Jinboxiang 1 walnut were selected,which laid the foundation for constructing rapid propagation and amplifying walnut planting scale.