黑木耳黄酮类化合物提取工艺研究

Study on Flavonoids Extraction from Auricularia auricula by Ethanol Extraction Method

[目的]研究乙醇浸提法提取黑木耳中黄酮类化合物的工艺。[方法]采用单因素试验和正交试验检测料液比、乙醇浓度、提取时间和提取温度对黑木耳黄酮类化合物提取得率的影响。[结果]最佳提取条件为:料液比1:7,乙醇浓度80%,提取时间6h,提取温度50℃,在该工艺条件下提取率达到0.43%。[结论]该工艺条件下提取率较高,适于提取黑木耳中黄酮类化合物。

[Objective] The study aimed to research the technology of flavonoids extraction from Auricularia auricula by ethanol extraction method.[Method] The factors that affect the extraction ratio were investigated by single factor and orthogonal experiments,including the liquid-to-solid ratio,the concentration of solvent,extraction time and extraction temperature.[Result] The optimum conditions were as follows：solid-liquid ratio was 1：7,ethanol concentration was 80%,extraction time was 6 h and temperature was 50 ℃.On this condition,the extraction ratio was 0.43%.[Conclusion] The process conditions were suit for flavonoids extraction from Auricularia auricula.