饥饿期粪肠球菌生物膜的体外培养实验

Experimental Study of Biofilms Formed by Starved Entercoccus Faecalis Cells in Vitro

目的体外培养观察饥饿期粪肠球菌的生物膜特点。方法通过菌落计数法计数,绘制生长曲线并找出饥饿期时间点,扫描电镜观察其生物膜特点。结果粪肠球菌在BHI液中的生长曲线可分为:迟缓期、对数生长期、稳定期、饥饿期,细菌在72h处于饥饿期。扫描电镜观察显示24h时形成的粪肠球菌生物膜厚度明显增加,菌细胞间可见胞外基质以及孔道。结论饥饿期粪肠球菌可在玻片上形成生物膜。生物膜在不同时间点经历从附着-聚积-成熟-播散的过程。

Objective To observe the structure of biofilms formed by the starvation phase of entercoccus faecalis （E. faecalis） in vitro. Methods The growth curve was made by CFU counts methods in different culture times to find the starvation phase. The biofilms were observed by SEM to compare the different stages. Results The growth curve of E. faecalis at BHI medium was divided into four phases： lag phase, exponential phase, stationary phase and starvation phase. Bacteria cultured at 72 h were the starved cells. The thickness of biofilms increased obviously, and the cells were packed by extracellular matrix and spiced with some channels after incubation for 24 h by SEM. Conclusion E. faecalis cells in starvation phase can form biofilms onto glass slices materials. The development of biofilms is divided into adsorptive-cumulate-mature-dispersive period.