大豆与疫霉菌非亲和互作早期差异显示基因的表达分析

Differential gene expression during early stage of incompatible interaction between soybean and Phytophthora sojae

大豆疫霉根腐病是大豆的毁灭性病害。为了深入了解大豆对疫霉菌的分子抗病机制,以大豆疫霉菌1号生理小种游动孢子接种抗性品种绥农10的根部及下胚轴,通过反转录差异显示技术分离到疫霉菌侵染0、0.5、1、2和4h后大豆下胚轴和茎部的差异表达基因,其中至少有8个基因与抗病相关。接种后0.5 h开始上调表达的有肉桂酸-4-羟化酶基因、ATP合成酶β亚基基因,以及类花生泛素结合酶基因;接种后1h和2h依次开始上调表达的有尿苷二磷酸-N-乙酰基-α-D-氨基半乳糖基因和豌豆蓝铜蛋白基因;接种后4 h才上调表达的有TGA型碱性亮氨酸拉链基因、大豆环孢素基因和14-3-3蛋白基因。这8个基因中有1个基因与信号传导有关、4个基因与抗病和防御有关、2个基因与转录调控有关、1个基因与能量代谢有关。研究表明,以上8个基因在疫霉菌游动孢子萌发、侵入大豆和在大豆体内扩展过程中起着重要作用。

Soybean root rot caused by Phytophthora sojae is a destructive disease.In order to understand molecular mechanisms of soybean resistance to Phytophthora sojae,the gene expression in hypocotyls and stems of variety ＇Suinong 10＇ were investigated by using mRNA differential display reverse transcription PCR at 0,0.5,1,2 and 4h after inoculation with zoospores of P.sojae race 1.The results showed that at least eight differential fragments at the transcriptional level were related to resistance genes.These fragments represented cinnamic acid 4-hydroxylase gene,Atp β gene coding ATP synthase β subunit and ubiquitin-conjugating enzyme gene which upregulated at 0.5h post inoculation,UDP-N-acetyl-alpha-D-galactosamine gene which upregulated at 1h post inoculation and blue copper protein gene which upregulated at 2h post inoculation,TGA-type basic leucine zipper protein gene,cyclophilin gene and 14-3-3 protein gene which upregulated at 4h post inoculation.The results suggested among these eight genes,one related to signal transduction,four related to resistance and defense,two related to transcription and regulation,and one related to energy metabolism.The results showed that aforesaid genes played important roles during zoospore germination,penetration and mycelium growth of P.sojae in soybean.