强碱性壳聚糖分离介质的制备及其性能研究

Preparation and Properties of a Chitosan Strong Basic Separation Media

利用反相悬浮交联法制备了壳聚糖基质,基质再与环氧丙基三甲基氯化铵反应,制得强碱性的壳聚糖阴离子分离介质。研究了分离介质的最佳制备条件,测定了其最大阴离子交换容量和滴定曲线,最后用5′-鸟苷酸对其进行交换性能表征。结果表明,最佳制备工艺为:以1.0%(m/V)的NaOH为催化剂,1.0g壳聚糖基质与5.0mL环氧丙基三甲基氯化铵于50℃反应3.0h,产物为强碱性阴离子分离介质,其最大交换容量为2.88mmol/g干介质,交换吸附结果表明对5′-鸟苷酸最大吸附量为98.59mg/g干介质。

The chitosan matrix previously made by the methods of inverse phase suspension and chemical crosslinking was then modified by glycidyl trimethylammonium chloride to prepare the strong basic anion separation media. The reaction conditions were optimized, the maximum exchange capacity, pH titration curves and the adsorption of 5＇- GMP of the media were studied. The optimal preparation conditions of the media were as followed： under the catalysis of 1.0 % （m/V）NaOH, 5. 0mL glycidyl trimethylammonium chloride reacted with 1.0g chitosan matrix at 50℃ for 3. 0 h. Under these conditions the maximum exchange was 2. 88mmol/g dry media. The results show that it was strong basic anion separation media by the pH titration curves, the study of adsorption indicated that the maximum attachment of 5＇-GMP was 98. 59mg/g dry media.