狭基线纹香茶菜低温压差式提取物对刀豆蛋白A所致肝损伤的保护作用

Protective effect of isodon lophanthoides on hepatic injury induced by Concanavalin A in mice

目的探讨狭基线纹香茶菜低温压差式提取物对刀豆蛋白A(Con A)所致小鼠肝损伤的保护作用。方法用Con A诱导小鼠肝损伤,用狭基线纹香茶菜低温压差式提取物灌胃治疗,一周后取血,测定血清ALT、AST活性,检测肝组织超氧化物岐化酶(SOD)的活性和丙二醛(MDA)及一氧化氮(NO)水平,同时测体重、肝脏重、脾重和胸腺重,计算脏器指数,取肝组织做病理组织学检查。结果狭基线纹香茶菜低温压差式提取物中、低剂量组能显著降低血清ALT、AST水平(P<0.05或P<0.01),高、低剂量组能显著升高血清SOD活性(P<0.05),高、中、低剂量组均能显著降低MDA及NO含量(P<0.01);病理检查结果显示狭基线纹香茶菜治疗组小鼠肝脏损伤程度减轻。结论狭基线纹香茶菜低温压差式提取物对Con A所致小鼠免疫性肝损伤有保护作用。

Objective To study the protective effects of Isodon lophanthoides var.gerardianus （Benth.） H.Hara （ILVG） aqueous extract on hepatic injury induced by Concanavalin A （Con A） in mice. Methods The model of hepatic injury induced by Con A was used for evaluating the therapeutic effects of ILVG in mice. After 6days of ILVG was given to mice by gastric perfusion, the levels of ALT and AST in serum were analyzed.Super oxide dismutase （SOD）activity,maleic dialdehyde （MDA） and nitric oxide （NO）content in hepatic tissue were determined. The body weight, liver weight, spleen weight and thymus weight of each mouse were measured. Liver tissue pathology was observed. Results Low and middle dosage group of ILVG decreased the ALT, AST in serum（P〈 0.05,P〈0.01）, low and high dosage group of ILVG increased SOD activity （P〈0.05）, low, middle and high dosage group of ILVG decreased MDA and NO content in hepatic tissue （P〈0.01）. ILVG restrained the enlargement of liver and spleen, reduced necrosis showed by pathological observation. Conclusion ILVG aqueous extract possesses the effects for protecting on acute hepatic injury induced by Con A in mice. It may be through the way of antilipoperoxidation and suppressing the release of NO.