黄山贡菊挥发油的抑菌及抗氧化性能研究

Study on the Bacteriostatic and Anti-oxidative Properties of Volatile Oil from Yellow Mountain Chrysanthemum Tea

通过水蒸气蒸馏法,提取黄山贡菊不同部位(根茎、花、叶)的挥发油,以4种细菌(大肠杆菌、变形杆菌、枯草杆菌、苏云金芽孢杆菌)和2种真菌(酿酒酵母、突变木霉)作为供试菌种,通过体外抑菌实验研究了黄山贡菊挥发油的抑菌性能。实验表明贡菊的挥发油对6种供试菌种均具有一定的抑制作用,特别是对4种细菌的抑制更加明显。从贡菊不同部位提取的挥发油其抑菌性能有较明显的差异,其中从黄山贡菊叶中提取的挥发油的抑菌性能最强。同时对从黄山贡菊叶中提取的挥发油进行了抗氧化性能的实验研究,结果表明黄山贡菊挥发油有很好的抗氧化能力,能够有效地清除羟自由基和脂过氧自由基,且在实验浓度范围内,随着挥发油浓度的升高其清除能力呈上升趋势。

Volatile oil is extracted from different parts（roots and stems,flowers,leaves） of yellow mountain chrysanthemum tea by steam distillation（SD）.The bacteriostatic property of its volatile oil is studied with four kinds of bacteria（Escherichia coli,Proteusbacillus vulgaris,Bacillus subtil and Bacillus thuringiensis） and two kinds of fungi（Saccharomyces cerevisiae and Trichoderma Vride G） as test subjects through extraneous bacteriostatic tests.The results show that its volatile oil has bacteriostatic action on all the six strains,and the action on the four kinds of bacteria is more evident than that on the two kinds of fungi.Besides,there is a remarkable difference in the bacteriostatic property of volatile oil from different parts of yellow mountain chrysanthemum tea.Volatile oil extracted from its leaves has the strongest bacteriostatic activity.Meanwhile,the anti-oxidantion capacity of volatile oil extracted from the leaves of yellow mountain chrysanthemum tea is studied.The results show that volatile oil extracted from its leaves has a good anti-oxidant capacity,and can effectively scavenge hydroxyl radicals and lipid peroxy radicals.In the experimental concentration range,its scavenging capacity rises with the increase of the concentration of volatile oil.