萝卜雄性不育系花蕾发育过程中内源激素分析

Analysis on the Changes of Phytohormones During Flower Bud Development in Male-sterile Radish

利用间接酶联免疫(ELISA)法测定了萝卜雄性不育株及可育株花蕾发育过程中内源激素含量的动态变化,以揭示萝卜雄性不育发生与其内源激素的关系。结果表明:在花蕾的发育过程中,不育株和可育株内源激素IAA、ABA、GA3、ZR以及JA含量变化均差异明显,在不育发生的初始阶段,不育株内源IAA含量上升32.09%,ABA含量下降75.45%,JA含量下降了11.20%,其余2种内源激素含量差异很小。IAA/ABA、IAA/ZR、IAA/GA3和GA3/ABA在二者间的变化趋势不一致,且比值大小差异很大,在不育发生的初始阶段,IAA/ABA、IAA/ZR和GA3/ABA平衡失调。表明内源激素可能与萝卜雄性不育的发生密切相关。

The changes of the contents of endogenous phytohormones were measured by ELISA（enzymelinked immunosorbent assay）to analyse the dynamic changes of endogenous phytohormones in different development stages of buds between male sterility and fertile radish.The results showed that the change of the phytohormones contents was significant during the whole bud development stages in male fertile and sterile plants at the development stage when the sterile phenomenon begin to appear,the content of IAA in male sterile bud was higher 32.09% than that in the normal fertile bud;while ABA and JA content were 75.45% and 16.18% lower than that of CK,respectively.The contents of the other two phytohormones change little.The change of IAA/ABA,IAA/ZR,IAA/GA3,GA3/ABA were inconsistent,and the discrepancy of them was significant,IAA/ABA,IAA/ZR and GA3/ABA showed inbalance when the sterile phenomenon began to appear,and suggested that endogenous hormones may be related to male sterility tightly in radish.