偃麦草属植物种间苗期抗旱性评价

Assessment of interspecies drought resistance of Elytrigia at the seedling stage

采用温室模拟干旱胁迫-复水法,对收集自6个国家的10种偃麦草属植物的种间苗期抗旱性进行综合评价,分别于干旱胁迫开始前(CK),连续干旱胁迫7,14,21,28,35d,以及复水7d后,对其叶片相对含水量(RWC)、相对电导率(REC)、脯氨酸含量(Pro)、丙二醛含量(MDA)、可溶性糖含量(SSC)5个抗旱生理指标,以及干旱胁迫到35d时复水7d后的植株平均存活率(ASR)进行测定分析,并采用欧氏距离法进行5个生理指标的综合聚类分析,可将10个植物种划分为3个抗旱等级,即相对抗旱的包括:ET001、EPU02、EJ003和EH001;中等抗旱的包括ES001、EE013和EC002;相对旱敏感的包括:ER037、EL001和EI022。

The drought resistance of 10 species of Elytrigia,collected from six countries,was identified and evaluated using simulated drought stress and rewatering in a green house at the seedling stage.The relative water content of leaves（RWC）,relative electrical conductivity rate（REC）,free proline accumulation（Pro）,contents of malondialdehyde（MDA） and the soluble sugar content（SSC） were measured at 0（CK）,7,14,21,28,and 35 days of drought stress and 7 days after rewatering.Their average survival rates（ASR） after 35 days of drought stress followed by the rewatering were calculated.The three drought resistant classes： high drought resistance（ET001,EPU02,EJ003 and EH001）,medium drought resistance（ES001,EE013 and EC002） and weak drought resistance（ER037,EL001 and EI022） were classified using the above 5 physiological indexes by the Euclidean distance clustering method.