环境因子对南极海洋芳香烃降解微生物生长和降解的影响

Influence of environmental factors on degradation of aromatic hydrocarbons and microorganisms growth in Antarctic sea

以从南极地区分离纯化的具有低温芳香烃降解功能的嗜冷菌Planococcus sp.NJ41为研究对象,模拟低温海洋环境对影响其生长和降解的环境因子进行分析研究,确定其最适的萘降解条件:动球菌Planococcus sp.NJ41可在以萘为唯一碳源的无机盐培养基中生长并在低温(0～10℃)下高效降解萘等芳香烃;其最适生长温度为10℃,最适pH为7.5,初始萘浓度在400 mg/L时NJ41具有最强的降解活性,最适N源为NH4NO3,摇床转速和微量元素均能对降解产生一定的影响。NJ41在最适条件下培养10 d时对萘的降解率最高可达48.8%,温度升高降解能力下降,超过25℃则基本不具有降解能力。

The present experiment,through a preliminary study of a cold-adapted PAHs-degrading strains psychrophile Planococcus sp.NJ41 which was screened from the Antarctic region,the simulation and analysis of influences of environmental factors in low-temperature environment on the growth and degradation were done.In the most suitable culture conditions established：NJ41 could grow in inorganic salt nutrient medium with PAHs such as naphthalene as the only carbon source and could degrade PAHs with high efficiency at low temperature（0~10℃）.The optimum growth temperature was 10℃,pH was 7.5,NJ41 had the highest degradation activity when the initial naphthalene concentration was 400 mg/L.The optimum nitrogen source was ammonium nitrate,and the rotation speed and trace elements may impact on the degradation.NJ41 had the highest degradability of naphthalene,48.8% at 10℃,120 r/min and 10 d,and the degradability of NJ41 was decreased with temperature up,but was not degradable above 25℃.