一株产黑褐色色素海洋放线菌的研究

Research on a marine actinomycete producing black-brown pigment

从舟山群岛潮间带海域中分离到一批产色素的海洋放线菌,用滤纸片法进行抑菌试验,并对目标菌株进行鉴定,然后对其所产色素进行粗提,测色素的最大吸收波长,研究理化因子对其影响。结果表明筛选到产黑褐色色素的菌株B2,其对Vibrio parahaemolyticus和Escherichia coli具有较好的抑制作用,色素初步研究结果显示,在波长456nm处具有最大吸收值,光照对色素稳定性影响较弱,不耐高温和酸碱。生理生化鉴定和16S rDNA分子序列分析显示菌株B2为链霉菌属的一种,系统进化树显示B2与菌株Streptomyces sp.28a-34-3具有最好同源性,相似性为99.8%。菌株B2具有较好的研究价值。

Some marine actinomycetes producing the pigment were isolated form the Zhoushan Islands in the intertidal area.The screening target was strained by the filter paper method and identified,then extracted the pigment,measured the maximum absorption wavelength and researched effect on physical and chemical factors.A marine actinomycete strain B2 producing back-brown pigment was isolated,and it has good inhibitory effect on Vibrio parahaemolyticus and Escherichia coli.The preliminary research showed that the pigment had the maximum absorption value at 456 nm,and there was weak effect of light on the pigment,neutral conditions and low temperature were good to its stability.16S rDNA sequences analyses,physiological and biochemical identification showed the strain belong to Streptomyces sp.Phylogenetic tree displayed B2 had the best homology with strain Streptomyces sp.28a-34-3,and their similarity was 99.8%.The strain B2 had good value for research.