pH及光照对两种赤潮甲藻种群生长和胞外碳酸酐酶活性的影响

Effect of pH and light on population growth and activity of extracellular carbonic anhydrase in two species of dinoflagellates

研究了pH和光照强度对两种赤潮甲藻(Prorocentrum donghaiense和Karlodinium micrum)的种群生长及其胞外碳酸酐酶(CA)活性的影响。结果表明,pH 7.5、光照强度30μmol/(m2.s)是两种微藻的最适种群生长条件,在种群生长的最适pH和光照强度下,胞外CA活性最大。最适培养条件下,两种甲藻胞外碳酸酐酶活性随着种群生长周期而变化,指数生长期至平台期CA酶活增加,平台期至衰退期CA酶活降低,平台期胞外CA活性最大。

The effects of pH and light intensity on population growth and extracellular carbonic anhydrase（CA） activity in two species of dinoflagellates（Prorocentrum donghaiense and Karlodinium micrum） were studied.The results show that pH 7.5 and 30μmol/（m2·s） irradiance which triggered the optimum population growth,were the conditions that favored the highest activity of extracellular CA in the two dinoflagellates.Under the optimum cultivation conditions,the extracellular carbonic anhydrase activity in the two dinoflagellates varies with the population growth phases.CA activities were increased from exponential phase to the stationary phase and decreased form stationary phase to the decline phase.The highest extracellular CA activity occurred at the stationary phase.The significance of the population growth related CA activity in population dynamics of the two dinoflagellates was also discussed.