离子色谱法测定水产品中亚硫酸盐的含量

Determination of Sulphite in Seafood by Ion Chromatography

采用阴离子色谱-抑制电导检测器测定虾仁等水产品中的亚硫酸盐(二氧化硫)的残留量,样品在碱性条件下经甲醛提取,超滤杯纯化,以KOH溶液为淋洗系统,抑制电流125 mA时检测。线性范围为0.1～40 mg/kg,相关系数为0.998 6,平均回收率范围为80%～98%。该方法检出限(S/N=3)为0.1 mg/kg,定量限为4.5 mg/kg。本方法快捷、简单、准确,适用于出口水产品中亚硫酸盐的测定。

A method was studied for determing residue of sulphite in seafood such as shrimps by anion exchange chromatography. Samples were absorbed with formaldehyde under alkaline condition, purified by centrifugal filter devices, detected in 125 mA suppressed current. The linear fit range was 0.1-40 mg/kg and the mean fortification recoveries were between 80% and 100%. The detection limit （S/N=3）for the method was 0.10 mg/kg and the determination limit was 4.Smg/kg. The method is suitable for detection of export seafood and needs the demand of food detection.