摘要
应用气相色谱-质谱联用技术(GC-MS)对微生物细胞脂肪酸的组成进行了分析。结果表明,菌体的代谢状态分为两个时期:菌体生长期和油脂积累期;菌体细胞主要积累油酸(C18:1)、软脂酸(C16:0)和棕榈油酸(C16:1);通过发酵,最终得到的微生物油脂脂肪酸组成为:油酸(C18:1)64.80%、软脂酸(C16:0)19.90%、棕榈油酸(C16:1)10.94%、硬脂酸(C18:0)3.72%和亚油酸(C18:2)0.56%。GC-MS可以很好地用于脂肪酸甲酯的分析,具有普遍的应用意义。
The composition of microorganism cellular fatty acid was analyzed by gas chromatography-mass spectrometry(GC-MS).The results showed that the metabolism of the strain could be divided into cell growth stage and fatty acid accumulation stage.T.cutaneum lipid was mainly comprised of oleic acid(C18:1),palmitic acid(C16:0),palmitoleic acid(C16:1).The fatty acid composition was as follows:oleic acid(C18:1)64.80%,palmitic acid(C16:0)19.90%,palmitoleic acid(C16:1)10.94%,stearic acid(C18:0)3.72% and linoleic acid(C18:2)0.56%,respectively.The fatty acid methyl esters could be well analyzed by GC-MS,which could also be generalized to many other applications.
出处
《化学与生物工程》
CAS
2011年第10期85-87,共3页
Chemistry & Bioengineering
基金
上海市浦江人才计划项目(08PJ14038)
国家重点实验室专项经费资助项目(2060204)
教育部留学回国人员科研启动基金资助项目
上海市重点学科建设项目(B505)
关键词
微生物细胞
油脂含量
脂肪酸组成
气相色谱-质谱联用技术
microorganism cell
lipid content
fatty acid composition
gas chromatography-mass spectrometry(GC-MS)