贮藏温度和时间对马尾松花粉保护酶活性及萌发率的影响

Effects of Storage Temperature and Storage Time on Germination Rate and Protective Enzymes Activity of Pinus massoniana Pollen

研究室温,4,-10和-20℃4种贮藏温度和0,72,144,216,286和358天6种贮藏时间对马尾松花粉萌发率及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的影响。结果表明:马尾松花粉的最佳贮藏条件为:室温72天,4℃216天,-10℃和-20℃286天,此时的花粉保持较高的萌发率和较强的抗氧化抗衰老能力;在贮藏过程中,3种酶出现最高峰值前后花粉萌发率出现大幅度下降;不同贮藏温度下,3种保护酶的作用不同,室温和4℃贮藏温度下,CAT为敏感型保护酶,SOD和POD为非敏感型保护酶;-10和-20℃贮藏温度下,POD为敏感型保护酶,SOD和CAT为非敏感型保护酶。

In this paper,I investigated impacts of four storage temperatures（room temperature,4 ℃,-10 ℃ and-20 ℃） and six storage times（0,72,144,216,286 and 358 days） on Pinus massoniana pollen germination and activities of SOD,POD and CAT.Results showed that P.massoniana pollen could remain a high germination proportion and strong resistance to oxidation for 72 days at the room temperature,216 days at 4 ℃ and 286 days at-10 ℃ or-20 ℃ respectively.During storage period,the germination proportion of pollen decreased significantly when the activities of SOD,POD and CAT were around the highest levels.The three enzymes had different responses to the storage temperature.CAT was a responding protection enzymes,but SOD and POD were non responding ones at room temperature or 4 ℃;however,POD was a responding protection enzymes,whereas SOD and CAT were non responding ones at-10 or-20 ℃.