麻疯树柄细蛾交尾行为及雄虫对性信息素粗提物的触角电位反应

Mating Behavior and Male EAG Response to Crude Extracts of Sex Pheromones from Female Stomphastis thraustica

在(25±1)℃、光周期16L:8D条件下研究麻疯树柄细蛾成虫的交尾行为、交尾节律及雄虫对性信息素粗提物的触角电位反应。结果表明:无论雌雄,成虫1日龄均可交尾,在1日龄时达到最高交尾率(70.12%),随着日龄的增加,交尾率降低,最低交尾率仅为5.81%,但交尾持续时间从89.33min增加到171.67min;在不同光照强度下,不同日龄成虫均能交尾,随着光照强度的增加,1日龄成虫交尾率从53.33%增加到69.40%;不同光强对4,5日龄成虫交尾持续时间影响不大;雄虫对性信息素粗提物的触角电位反应表明:在正己烷、正戊烷、二氯甲烷和乙醚4种溶剂中,正己烷是提取性信息素效果最好的溶剂;随着雄蛾日龄的增加,其对性信息素粗提物的反应逐渐下降;腺体提取比空气提取效果好;雄蛾对性信息素粗提物的反应最低浓度为10-3FE·(10μL)-1。

Stomphastis thraustica is a serious leaf miner in Jatropha curcas in this study,we investigated adult mating behavior,mating rhythm and male EAG response to crude extracts of sex pheromones from female S.thraustica at（25±1）℃ with 16L：8D light period.The results showed that both female and male of 1-day-old adults could mate,and the highest mating frequency reached to 70.12% when the adults were all 1-day-old.The mating frequency decreased with increase of adults＇ age.The lowest mating frequency was only 5.81%.However,the mating duration increased from 89.33 min to 171.67 min with increasing adults＇ age.Under the different illumination intensity,adults could mate regardless of the daily age.However,the mating frequency of 1-day-old adults increased from 53.33% to 69.40% with illumination strengthening.However different illumination intensity had not significant influence on mating duration of 4 and 5-day-old adults.The experiment of male EAG response to sex pheromones crude extracts showed that the hexane was the best solvent for extracting female sex pheromone among hexane,pentane,dichloromethane and diethyl ether.With the increase of male adults＇ daily age,the male EAG response to sex pheromones crude extracts decreased.The crude extracts from female sex pheromone glands were more active than that obtained by air collection.The lowest concentration of sex pheromones for consistent male EAG response was 10-3 FE·（10 μL）-1.