虎榛子根际真菌对油松幼苗的化感作用

Rhizosphere Fungi of Ostryopsis davidiana and the Allelopathic Effects on Piuns tabulaeformis

对分离自虎榛子根际的几株真菌,通过液体纯培养法获得其代谢产物,设计不同浓度后施加到播种60天后的油松苗基质中,探讨虎榛子根际真菌对油松的化感效应。结果表明:拟青霉菌属的菌株(P3)和葡萄穗菌属菌株(S1)对油松幼苗生长具有显著的促进效应,并随着浓度的增大其作用增强;相反,青霉菌属的菌株(P1)对油松苗具有显著的抑制作用;胶帚霉属菌株(G1)和青霉菌属的菌株(P2)表现出低浓度促进、高浓度抑制的效应。有促进作用的菌株(P3、S1)均含有较高的油酸甲酯、9-油酸(E)、9,12-十八碳二烯酸(Z,Z)、9-十八烯酸酰胺、六氢化吡咯并(1,2-α)吡嗪-1,4-二酮等化合物,而具有抑制作用的P1菌株则含有较高的番茄黄素和大戟二萜醇。

In this paper some dominant rhizosphere fungi of the Ostryopsis davidiana were collected and its metabolites were extracted from the liquid pure culture.The metabolites in different concentrations were added to the culture medium for 60-day-old seedlings of Piuns tabulaeformis to investigate allelopathic effects that rhizosphere fungi of Ostryopsis davidiana might have on Piuns tabulaeformis.The results showed that the strain P3 of Paecilomyces and strain S1 of Stachybotrys significantly promoted seedling growth,and the effect was strengthened as the concentration increased.However,strain P1 of Paecilomyces obviously inhibited seedling growth.Strain G1 of Gliocladium and strain P2 of Paecilomyces showed effects of promoting seedling growth in low concentrations,and prohibiting seedling growth in high concentrations.On the base of allelopathic effects study,we chose three strains which had obvious effects on the Piuns tabulaeformis,i.e.P3,S1,P1,and then cultivated them in liquid pure culture method.The chemical productions were analyzed by GC/MS method,and compounds,such as oleic acid,methyl ester;9-octadecenoic acid（E）;9,12-octadecadienoic acid（Z,Z）;9-octadecenoamide;hexahydropyrrolo（1,2-α） pyrazine-1,4-dione were detected in the promoting strains（P3,S1）,and lycoxanthin and euphorbiae diterpene glycol were found in inhibiting strain（P1）.