血清脂联素和IL-10检测在新生儿缺氧缺血性脑病中的诊断价值

Diagnostic value of serum adiponectin and interleukin-10 in newborn infants with hypoxic-ischemic encephalopathy

目的探讨新生儿缺氧缺血性脑病(HIE)患者血清脂联素和白细胞介素10(IL-10)变化的临床意义。方法采用双抗体夹心酶联免疫吸附法(ELISA)检测60例足月HIE患儿(轻度11例、中度33例、重度16例)及30例健康足月新生儿(健康对照组)出生24h内血清脂联素和IL-10的水平,比较各组间的差异;并分析HIE各组中血清脂联素和IL-10的相关性。结果 HIE组血清脂联素水平低于健康对照组(P<0.01);HIE各组脂联素水平随HIE加重而降低,各组间差异均有统计学意义(P<0.01);HIE组血清IL-10水平高于健康对照组(P<0.01);HIE各组IL-10水平随HIE程度加重而增高,各组间差异均有统计学意义(P<0.01);HIE各组血清脂联素和IL-10呈显著负相关(r分别为-0.728、-0.804、-0.815,P<0.05)。结论血清脂联素和IL-10水平可作为HIE病情严重程度和疗效判断的早期指标。

Objective To explore the changes and clinical value of serum adiponectin and interleukin-10 in newborn infants with hypoxic-ischemic encephalopathy（HIE）.Methods 60 newborn infants（HIE group）,including 11 cases as mild HIE group,33 cases as moderate HIE group and 16 cases as severe HIE group,and 30 cases healthy newborn infants（control group） were enrolled and detected for serum adiponectin and interleukin-10 within 24 h after born by using ELISA. The differences between the groups and correlation between serum adiponectin and interleukin-10 in HIE groups were analyzed.Results Serum level of adiponectin in HIE group was lower than that of control group（P0.01） and decreased with the severity of HIE in HIE subgroups （P0.01）. Serum level of interleukin-10 in HIE group was higher than that of control group（P0.01） and increased with severity of HIE in HIE subgroups（P0.01）.Serum level of adiponectin was negatively correlated with that of interleukin-10 in HIE subgroups （r=-0.728、-0.804、-0.815,P0.05）.Conclusion Serum adiponectin and interleukin-10 could be used as criteria for judging disease severity and observing therapeutic effects at early phase of HIE.