4种尿微量蛋白联合检测对2型糖尿病肾病改变的临床诊断的应用评价

Diagnostic significance of combined detection of 4 urinary trace proteins for early diagnosis of renal damage of type 2 diabetes mellitus

目的进行4种尿液微量蛋白:尿转铁蛋白(UTRF)、尿免疫球蛋白G(UIgG)、尿微量清蛋白(UmALB)、尿α1-微球蛋白(Uα1-M)的联检,观察其在2型糖尿病(T2DM)肾病改变的变化,探讨其临床诊断价值。方法采用速率散射比浊法测定UTRF、UIgG、Uα1-M、UmALB的浓度,分析其在T2DM肾病改变中的变化。结果 T2DM肾病改变组的4种尿液微量蛋白UTRF、UIgG、Uα1-M、UmALB测定结果的中位数、数据范围,较T2DM无肾病改变组有明显增高;各项指标两两比较的秩和检验结果显示:P=0.00,差异有统计学意义。结论 4种尿液微量蛋白的联检,可有效提高对T2DM肾病改变的检出率,在其早期诊断、及时治疗、病程监测、预后判断中具有临床应用价值。

Objective To investigate the diagnostic value of combined detection of urinary trace proteins,including albumin（UmAlb）,α1-microglobulin（Uα1-M）,IgG（UIgG） and transferrin（UTRF） for renal damage in patients with type 2 diabetes mellitus（T2DM）.Methods UIgG,UmAlb,Uα1-M and UTRF were detected by using with immunoturbidimetry and changes of the levels of 4 proteins were analyzed.Results Median and range of UmAlb,UIgG,Uα1-M and UTRF in patients with renal damage were higher than those without renal damage （P=0.00）.Conclusion Combined determination of UmAlb,Uα1-M,UIgG and UTRF could be used as sensitive method for the diagnosis of early renal damage in patients with T2DM,and provide reliable proof for monitoring injury extent and observing therapeutic effects.