诱变与原生质体融合联合选育异抗坏血酸发酵菌株的研究

Screening of strains produing erythorbic acid by mutagenesis and protoplast fusion

采用诱变与原生质体融合技术联合筛选高产异抗坏血酸发酵菌株。通过紫外诱变与硫酸二乙酯诱变对出发菌株FZ-13进行诱变选育，再利用具有抗药性标记的亲本进行原生质体融合筛选出高产且遗传稳定性好的异抗坏血酸发酵菌株。通过诱变筛选出了产量比FZ-13提高200％-473％的27株青霉菌株；通过原生质体融合试验，筛选到产量比FZ-13高563．7％的菌株L11，L11的产量最高为6．93mg／mL，且遗传性状稳定。诱变能有效提高异抗坏血酸发酵菌株的产量，同时原生质体融合技术也可用来提高异抗坏血酸发酵菌株的产量。

The strains producing high yield of erythobic acid were screened with mutagensie and prolast fusion. The strain FZ-13 was mutagenized by ultraviolet rays and ethyl sulfate. The high erythobic acid production strains were further screened. The good strains were selected for protoplast fusion. After mutagenesis, 27 strains were screened and their erythorhic acid production was increased by 2.0-4.7 folds compared with that of the strain FZ 13. After protoplast fusion, the fusion L11 was obtained and its yield of erythorbic acid was 6.93mg/ml, which was 563.7% higher than that of the strain FZ13. L11 had the highest yield of erythorbic acid and its heredity was stable. The results indicated that mutagenesis was an effective technique to enhance erythorbic acid production and protoplast fusion could also improve the yield of erythorbic acid.