纳米TiO_2、ZnO、SiO_2对剑尾鱼肝组织中Na^+/K^+-ATP酶活性的影响

Effects of nanoparticle of TiO_2,ZnO and SiO_2 on Na^+/K^+-ATPase activity in the liver of Xiphophorus helleri

采用浸浴法研究了氧化纳米颗粒TiO2、ZnO、SiO2对剑尾鱼(Xiphophorus helleri)肝中Na+/K+-ATP酶活性的影响。结果表明:纳米TiO2处理组,高浓度(5 mg/L、10 mg/L)组表现为抑制作用,其中5 mg/L处理组Na+/K+-ATP酶的活性与对照组无显著差异(p>0.05),10 mg/L处理组中Na+/K+-ATP酶的活性显著低于对照组中酶的活性(p<0.05)。低浓度组(0.1 mg/L、1 mg/L)则表现为先诱导后抑制,除0.1 mg/L组在暴露1 d后与对照组有显著差异外(p<0.05),其余组与对照组均无显著差异(p>0.05)。纳米ZnO、SiO2处理组(0.1 mg/L、10 mg/L)在暴露1 d后,肝中Na+/K+-ATP酶的活性均比对照组高,随着暴露时间增加至20 d,Na+/K+-ATP酶活性下降,且显著低于对照组(p<0.05)。3种纳米颗粒的浓度为0.1 mg/L时,对暴露后1 d剑尾鱼肝中的Na+/K+-ATP酶活性的影响均为诱导作用,诱导大小顺序为ZnO>TiO2>SiO2;随着暴露时间的增加至10 d,纳米TiO2、ZnO、SiO2处理组对Na+/K+-ATP酶活性均表现出抑制作用。

The effect of oxide nano-particles TiO2,ZnO and SiO2 on the activity of Na＋/K＋-ATP enzyme in liver tissue of Xiphophorus helleri Heckel was studied by addictive indexmethod.The results showed that the activity of Na＋/K＋-ATP enzyme in the liver tissue was significantly higher than that of the control group（p0.05） by treatment of（0.1 mg/L and 1 mg/L） nano-particle TiO2 and 1d exposure.But when the exposure time was increased to 10d,the activity of Na＋/K＋-ATP enzyme was lower than that of control group,but with no significant difference（p0.05）;the activity of Na＋/K＋-ATP enzyme in the liver tissue was significantly higher than that of the control group（p0.05） by treatment of（0.1 mg/L and 1 mg/L） nano-particles ZnO and SiO2 after 1d exposure,but when the exposure time was increased to 20 d,the activity of Na＋/K＋-ATP enzyme was decreased and lower than that of the control group;treatments of 0.1 mg/L nano-particle TiO2,ZnO and SiO2 and 1d exposure,all treated nano-particles exerted inductive function towards the activity of Na＋/K＋-ATP enzyme in the liver tissue,and the induced effect were followed in this order： ZnOTiO2SiO2,when the exposure time was increased to 10d,all nano-particles TiO2,ZnO and SiO2 treated groups exhibited inhibitory effect towards the activity of Na＋/K＋-ATP enzyme in the liver tissue.