RP-HPLC测定小儿清热止咳颗粒中盐酸麻黄碱的含量

Determination of Ephedrine Hydrochloride inXiao'er Qingre Zhike Granules by RP-HPLC

目的:测定小儿清热止咳颗粒中盐酸麻黄碱的含量。方法:用水蒸汽蒸馏法提取小儿清热止咳颗粒中的麻黄碱;采用LUNA C18色谱柱(150×4.6 mm,5μm),流动相为乙腈-0.02mol/L KH2PO4溶液(含0.2%三乙胺,H3PO4调PH2.7)(3∶97);检测波长:210nm。结果:盐酸麻黄碱在0.08024～0.40120μg范围内呈良好线性关系,r=0.9999,平均回收率为97.6%,RSD为1.0%(n=6)。结论:该方法简便准确,重现性好,可作为控制药品质量的定量依据。

Objective： To establish a HPLC method for the determination of ephedrine hydrochloride contents in Xiao＇er Qingre Zhike granules.Methods： Extracted by steam distillation ephedrine in Xiao＇er Qingre Zhike granules;Separation was performed on LUNA C18 column（150×4.6 mm,5μm）.acetonitrile-0.02mol/L Potassium dihydrogen phosphate solution（containing 0.2%triethylamine,phosphoric acid adjusted PH2.7）（3∶97）was selected as the mobile phase,detection wavelength was 210 nm.Results： The linear range for ephedrine hydrochloride was 0.08024～0.40120μg（r=0.9999）,the average recovery of ephedrine hydrochloride was 97.6%,RSD=1.0%（n=6）.Conclusion： The method is simple,accurate and repeatable,and it can be applied to control the quality of this drug.