基于稀土复合纳米粒子的新型荧光共振能量转移体系的建立及研究

Study of a Novel Efficient FRET System Base on a Terbium Composite Nanoparticles as Donor

采用简便的超声合成方法,成功的制备了稀土复合纳米粒子Tb/acety acetone(acac)/Polyacrylamide(PAM).该复合纳米粒子具有较高的稳定性,良好的分散性.基于该稀土复合纳米粒子为供体,罗丹明B为受体,建立了一种新型高效的荧光共振能量转移(FRET)体系.实验结果表明该供体-受体对之间具有较高的能量转移效率.

A hightly efficient ultrasonic-assisted method was successfully applied to prepare the strong fluorescence Tb/acetyl acetone（acac） Polyacrylamide（PAM） composite nanoparticles.The nanoparticles are steady fluorescence and highly resistant to photobleaching.A novel fluorescence resonance energy-transfer（FRET） system with Tb/acac/PAM as donor and Rhodamine B as acceptor was set up efficiently.