唾液葡萄糖与血糖个体相关性的研究

An experimental research of the individual correlation between saliva glucose and blood glucose

目的探讨不同个体唾液葡萄糖和血糖值的相关性及重复性,为制定血糖个性化监测化方案奠定基础。方法选取临床确诊糖尿病患者为研究对象,用自制2,4,6-三溴-3-羟基苯甲酸为显色底物,在Hitachi-7150上测定不同个体餐前唾液葡萄糖与血糖浓度,计算不同个体两者的关系数。结果不同糖尿病患者唾液葡萄糖与血糖相关系数有较大差别,餐前相关系数平均为0.85,餐后相关系数平均为0.79,餐前相关系数3个月内的变异系数CV<5%。结论糖尿病患者餐前唾液葡萄糖浓度与血糖浓度相关系数较高,而且比较稳定,可用餐前唾液葡萄糖浓度替代血糖浓度,开展无创快速检测。

Objective To explore the individual correlation between saliva glucose and blood glucose and its reproducibility to establish foundation for formulate individual monitoring scheme of blood glucose.Methods Patients with definitely diagnosis of diabetes mellitus were enrolled and the levels of fasting saliva glucose and fasting blood glucose were detected on Hitachi7150,using self-made 2,4,6-tribromo-3-hydroxybenzoic acid（TBHBA） as chromogenic substrate.The correlation coefficient between saliva and blood glucose were calculated for every subjects.Results The individual correlation coefficients between saliva glucose and blood glucose varies greatly between different patients.The average fasting correlation coefficient was 0.85,the average postprandial correlation coefficient was 0.79 and the coefficient of variability of fasting correlation coefficient was less than 5% within three months.Conclusion The individual fasting correlation coefficient between saliva glucose and blood glucose could be relatively high and stable.Fasting blood glucose could be replaced by fasting saliva glucose for the noninvasive and fast detection.