保存前后滤除WBC的RBC悬液质量的对比性研究

Quality comparison of red blood cells suspension with filtering of white blood cells at different times

目的探讨保存前后滤除WBC(滤白)的RBC悬液质量的变化,为临床选择滤白时机提供参考依据。方法对4℃保存5d的悬浮RBC 20袋(每袋2U)用血库型滤器滤白作为保存后滤白组(A组),由血站滤白的少白悬浮RBC 20袋(每袋2U)作为保存前滤白组(B组);分别在第7天、第14天、第21天、第28天和第35天测定两组血细胞指标、炎性细胞因子水平。结果 B组血浆游离血红蛋白(FHb)在第14天明显升高,K+浓度在21d显著升高,且二者浓度随保存时间的延长而增加(P<0.05);A组炎性细胞因子(IL-1、IL-6、IL-8、TNF-α)水平在滤白后第21天时才明显升高,与B组比较差异有统计学意义(P<0.05);A、B两组回收率差异无统计学意义(P>0.05)。结论保存前后滤白都能达到很好的滤除效果,但不同的滤白贮存方式对血液的质量产生不同的效果,为临床选择不同的滤白RBC提供了实验依据。

Objective To investigate the changes of the quality of red blood cells（RBC） suspension with filtering of white blood cells（WBC） before and after storage to provide a reference for clinic to select the time for filtering of WBC.Methods 20 bags of suspension of RBC（2U for each bag）,saved for 5 days at 4 ℃,filtered out WBC with filters used in department of blood transfusion,were enrolled as group A.20 bags of RBC suspension（2U for each bag）,without storage and being filtered out WBC in Blood Center,were enrolled as group B.Blood samples of group A and B were detected for blood cells and the levels of inflammatory cytokines,and cultured for bacterial detection,at the 7th,14th,28th,21st and 35th day after WBC were filtered.Results In group B,the concentration of free hemoglobin（FHb） and K＋ increased at the 14th and 21st day,and gradually increased with the extension of storage time（P0.05）.In group A,the levels of inflammatory cytokines,including interleukin-1,interleukin-6,interleukin-8 and tumor necrosis factor-α,increased at the 21st day,and had statistical difference,when being compared with group B（P0.05）.There was no significant difference between A and B groups for recovery rate（P0.05）.Conclusion Filtering of WBC,whether before or after storage,could both have fine filtering efficiency,but different filtering ways might have variant effects on the quality of RBC suspension,which could provide experimental base for the selection of RBC suspension filtered out WBC.