骨髓间充质干细胞移植大鼠缺血心肌后GATA-4mRNA的表达

Expression of GATA-4 mRNA in transplanted MSCs ischemic myocardium in rats

目的观察骨髓间充质干细胞(MSCs)移植于大鼠缺血心肌后,心脏早发育基因GATA-4 mRNA的表达。方法设模型组、假手术组和空白对照组,其中模型组采用"冠脉结扎法"建立大鼠急性心肌梗死(AMI)模型。收集从SD雄性大鼠胫骨和股骨骨髓中分离、培养、鉴定后的MSCs,密度调至3×106/100μL悬液,移植于缺血心肌边缘组织的3个位点直接注入共100μL的MSCs细胞悬液,假手术组和空白对照组均在与模型组相同注射部位的3个位点直接注入共100μL的MSCs细胞悬液,28 d后处死大鼠进行相关实验样本处理。采用逆转录聚合酶链反应(RT-PCR)技术检测心肌组织中GATA-4 mRNA的表达。结果 MSCs移植模型组和假手术组的大鼠心肌中存在GATA-4 mRNA的表达;而空白组的心肌中未检出GATA-4 mRNA的表达,对其IOD值半定量分析,模型组高于假手术组和空白组,差异有统计学意义(P<0.05或<0.01)。结论 MSCs在进入缺血心肌环境后,细胞内的心肌特异转录过程被再次激活,确立了MSCs向心肌细胞分化的方向。

Objective To observe the expression of genes GATA-4 mRNA in the early heart development after bone marrow mesenchymal stem cells（MSCs） were transplanted into ischemic myocardium.Methods The model group,sham group and control group were set,and the acute myocardial infarction（AMI） model was established according to ＂coronary occlusion＂ method.,MSCs were collected the isolated,cultured and identified marrow from the tibia and femur of SD male rats,the density of MSCs was adjusted to 3×106/100 μL and then to be transplanted into three points of ischemic myocardium edge in model group and be injected 100 μL,and in sham group and control group were injected 100 μL of MSCs suspension into the same places.The rats were killed after 28 days to get the test samples,and then the myocardial expression of GATA-4 mRNA was detected wit reverse transcription polymerase chain reaction（RT-PCR）.Results The GATA-4 mRNA expression existed in model group and shaml group;but did not in control group,while the semi-quantitative analysis of IOD values showed that the expressions in model group were higher than those in other two groups（P〈0.01 or P〈0.05）.Conclusion After MSCs goes into the environment of ischemic myocardium,the transcription process of cardiac-specific was activated once again,which established the differentiation direction of MSCs to myocardial cells.