水提与醇提对竹子中愈创木酚含量的影响

Effects of water and ethanol-extraction of bamboo on guaiacol content

目的优选竹子中有效成分愈创木酚的最佳提取溶媒。方法将新鲜竹子砍成小段,再粉碎成粗粉,加水或70%乙醇回流提取,提取液采用高效液相法测定愈创木酚含量。色谱条件为:流动相0.5%H3PO4的水溶液-乙腈(78∶22);固定相为C18柱;流速1.0 mL/min;柱温20℃;检测波长220 nm。结果醇提法愈创木酚含量略高于水提法,但无明显差异。结论水提法可以代替醇提法。

Objective To optimize the best extraction solvent of effective ingredients in bamboo.Method The fresh bamboo was cuted into small pieces,then shattered into thick powder,add water or 70% ethanol reflux extraction.The guaiacol content in extract was assaied by HPLC method.Chromatographic conditions： The mobile phase was 0.5% triethylamine and 0.5% H3PO4 solution-acetonitrile（78：22）,stationary phase for using C18 column,flow velocity was 1.0 mL/min,column temperature was 20 ℃,detection wavelength was 220 nm.Result Alcohol formulation is slightly higher than the water extract method,but there is no obvious difference.Conclusion Water extract method can take the place of the formulation of alcohol.