芽孢杆菌制剂对蛋鸡生产性能、血清指标及盲肠微生物的影响

Effect of Bacillus Preparation on performance,Serum related Index,Cecal Flora of Layers

为研究芽孢杆菌制剂对蛋鸡生产性能、血清相关指标及盲肠微生物的影响。试验选择健康、产蛋均匀的259日龄的"京粉一号"蛋鸡3600只,随机分为2组,每组6个重复,每个重复300只鸡。对照组饲喂基础日粮,试验组在基础日粮中添加3%的芽孢杆菌制剂,进行为期40d的试验。试验第41天晨饲前,从每个重复随机选取中等大小的蛋鸡3只,翅静脉采血备测血清指标;同时将其麻醉后剖腹,用细绳分别结扎两只盲肠的开口端,剪下盲肠,用于测定盲肠内容物中的大肠杆菌和乳酸菌数。结果表明:①日粮中添加芽孢杆菌制剂,对其生产性能各指标无显著影响(P>0.05);②芽孢杆菌制剂对蛋鸡血清尿素氮、血糖、胆固醇等指标影响差异不显著(P>0.05),可以显著提高蛋鸡血清中总超氧化物歧化酶(SOD)的含量(P<0.05);③与对照组相比,试验组蛋鸡盲肠内乳酸菌增加5.19%(P>0.05),大肠杆菌数显著降低6.25%(P<0.05)。因此,在蛋鸡日粮中添加芽孢杆菌制剂能提高动物的抗氧化能力,改善肠道微生态结构。

This experiment was conducted to investigate the effects of Bacillus preparation on performance,serum related index,cecal flora in layers.Three thousand and six hundred 259-day-old ＇jing fen yi hao＇ layers were randomly divided into 2 groups,with 6 replications per group and 300 layers each replication.Layers in control group were fed basal diet.The trail groups were fed diet supplemented with 3% Bacillus preparation.The experiment lasted for 40 d.On day 41,three layers was selected from each replication,blood from wing vein were obtained and used to determine serum related index,then a layer were slaughtered,cecal was collected to determine contents of Escherichia coli and Lactobacillus.The results showed that：①there was no significant effect of Bacillus preparation on performance of layers;②there was no significant effect of Bacillus preparation on serum related index,except that the content of serum superoxide dismutase（SOD） was significantly improved（P0.05）;③the diet added with Bacillus preparation significantly reduced the contents of Escherichia coli in cecal（P0.05）,and increased the contents of Lactobacillus in cecal（P0.05）.It was concluded that supplementation of Bacillus preparation in diets increased the antioxidant capacity and improve intestine microbial flora of layers.