鹅大肠杆菌的分离鉴定及药敏试验

Separation,Identification and Drug Sensitive Test of Escherichia coli from Goose

为了对鹅大肠杆菌的临床治疗提供指导并为制备大肠杆菌自家灭活疫苗奠定基础,采集疑似病例的病料,用麦康凯培养基分离细菌,对分离的细菌进行形态学鉴定、生化鉴定、血清型鉴定、致病性试验及药敏试验。结果显示,所分离到的菌落呈粉红色、光滑湿润、边缘整齐;革兰氏染色为阴性小杆菌,两端钝圆,多单个存在;共鉴定出15株大肠杆菌,其中4株O35型,3株O78型,3株O26型,2株O7型,1株O109型,1株O141型,1株O2型;动物试验表明所鉴定的菌株对小鼠和雏鹅均有较高的致死率;体外抑菌试验结果发现强力霉素、阿莫西林对该场的鹅致病性大肠杆菌具有较强的抑制作用。本研究为有效防制鹅大肠杆菌病提供了理论依据,对于控制规模化鹅场大肠杆菌等细菌性疾病具有重要的应用价值。

In order to direct the treatment of goose E.coli disease and establish the foundation of preparation for the goose E.coli inactivated vaccine.The suspected cases of the goose E.coli disease were taken and separated and cultivated in MacConkey medium.The bacteria strains were obtained,and identificated by morphological assay,biochemistry assay,serotype assay,and animal experiments.Moreover,the identified E.coli were carried out by drug susceptibility test.The results showed that the strains of E.coli were pink,moist,smooth and neat in MaConkey medium,which were small,negative of Gram＇s staining,ends obtuse and many individual exists.The isolated bacteria were identified as E.coli,which animal experiment showed that the goose E.coli had higher infection rate and fatality rate.The 15 strains were identified by serotype assay： 4 O35 strains,3 O78 strains,3 O26 strains,2 O72 strains,1 O109 strains,1 O141 strain,1 O2 strain.In vitro antibacterial experiment results showed that Doxycycline and Amoxicillin against the above E.coli had strong inhibited effect.The research provide the theoretical foundation and significant applical value for efficientively preventing and curing goose E.coli disease.