哈蟆油水溶性总蛋白抗疲劳活性的研究

Study on the anti-fatigue function of water-miscible total proteins from Oviductus Ranae

研究了哈蟆油水溶性总蛋白的抗疲劳药理活性。采用中性缓冲液浸提,硫酸铵沉淀法制备哈蟆油水溶性总蛋白。设立哈蟆油水溶性总蛋白低、中、高三个剂量组(0.075、0.15、0.30g/kg),同时设立空白对照组,小鼠灌胃给药30d。观察小鼠体重、负重游泳时间、尿素氮含量、肝糖原含量、血乳酸含量等指标的变化。实验结果表明,哈蟆油水溶性总蛋白可延长小鼠负重游泳时间,降低血清尿素氮含量,显著增加肝糖原含量,显著降低血乳酸含量,对小鼠的体重无明显影响。证明了哈蟆油水溶性总蛋白具有一定的抗疲劳作用。

The pharmacological activity of water-miscible total proteins from Oviduetus Ranae was studied.Prepared water-miscible total proteins by neutral buffer extraction and ammonium sulfate precipitation.Low,medium,high（0.075,0.15,0.30g/kg）dose of water-miscible total proteins and a blank dose had been applied to mice for 30d continuously.The change of body weight of mice,swimming time,urea nitrogen,hepatic glycogen,and blood lactic acid content were observed.The results showed that the water-miscible total proteins of Oviductus Ranae could extend the swimming time of mice,significantly increase liver glycogen contents,significantly lower blood lactate levels,and had no significant effect on body weight of mice.The results proved the water-miscible total proteins of Oviductus Ranae had anti-fatigue effect.