灰叶丛枝病病原的分子鉴定及系统发育分析

The Molecular Identification and Phylogeny of Tephrosia Witches'-broom Phytoplasma

实验应用PCR扩增i、PhyClassifer分析、序列同源性及系统发育分析等方法对海南省灰叶丛枝病病害进行了分子检测及其病原的鉴定、系统进化研究。iPhyClassifer结果显示:灰叶丛枝病植原体为花生丛枝组(16SrⅡ组)16SrⅡ-A成员。序列同源性分析结果表明:灰叶丛枝病植原体与16SrⅡ、16SrⅡ-A成员同源性最高,如与花生丛枝病植原体16S rDNA的序列同源性为99.9%r,p基因为99.9%。基于16S rRNA和rp两基因构建的系统发育树显示:灰叶丛枝病植原体与花生丛枝病植原体、甘薯丛枝病植原体起源相同,且关系相当接近。根据以上结果,灰叶丛枝病植原体归为花生丛枝组(16SrII组)16SrⅡ-A亚组,与Candidatus Phytoplasma australasiae候选种相关。

The tephrosia witches＇-broom phytoplasma collected from Hainan province was classified and identified by sequencing techniques and iPhyClassifer. The results of sequencing and homologuous comparison with other phytoplasmas showed that tephrosia witches＇-broom phytoplasma shared 99.9% similarity with peanut witches＇- broom phytoplasma in 16S rRNA gene and 99.9% in rp gene. Tephrosia witches＇-broom phytoplasma are sorted into Peanut witches＇ -broom group （ 16Sr Ⅱ ）, 16Sr Ⅱ-A, according to the results of iPhyClassifer of 16S rDNA, phylogenetic analysis of 16S rRNA and rp gene, which is related to Ca. Phytoplasma australasiae.