微量元素锌、铜与体外受精-胚胎移植临床结局的关系

Relationship between the zinc and copper concentration and the clinical outcomes of in vitro fertilization and embryo transfer

目的探讨微量元素锌、铜元素与体外受精-胚胎移植（IVF-ET）临床结局的关系。方法回顾性分析43例IVF—ET治疗周期的临床资料以及绒毛膜促性腺激素（hCG）注射日血清和取卵日卵泡液内锌、铜元素含量。结果应用Pearson相关分析显示，在43例治疗周期中卵泡液内铜浓度与获卵数、受精数和卵裂数呈正相关关系（r值分别为0．392、0．327和0．321，P值分别为0．0094、0．0325和0．0361）。临床妊娠组与未妊娠组相比，卵泡液内锌、铜元素浓度差异均无统计学意义（P均〉0．05）；hCG注射日血清雌二醇差异有统计学意义[（4513．65±2538．3）ng／L与（6495．29±3402．8）ng/L，t=2．177，P=0．0353]；hCG注射日血清锌含量差异有统计学意义[（98．60±18．08）μmol／L与（87．89±10．61）μmol／L，t=2．153，P=0．0372]。结论微量元素锌、铜在IVF-ET过程中可能发挥着重要作用。

Objective To investigate the relationship between the zinc and copper concentration and the clinical outcomes of in vitro fertilization and embryo transfer （ivf-et）. Methods The clinical data and zinc and copper concentration of serum on the days with human Chorionic gonadotropin（hCG） injection as well as that in the follicular fluid on the the day of oocyte retrieval were retrospectively analyzed in 43 cases with IVF-ET cycles. Results Application of pearson correlation analysis showed that copper concentration in follicular fluid of the 43 cases was positively correlated with the numbers of retrieved oocytes, fertilized oocytes and cleaved embryos（ r were 0. 392, 0. 327 and 0. 321, respectively, P were0. 0094, O0. 0325 and 0. 0361 respectively） Between the pregnant group and non-pregnant group, the zinc and copper concentration in follicular fluid was not significantly different （ P 〉 0. 05 ） ; the serum estradiol （ E2 ） on the day of hCG administration was of significantly different（ t = 2. 177, P = 0. 0353 ） ; the serum zinc concentration on the day of hCG administration was of significantly different（ t = 2. 153,P = 0. 0372）. Conclusion Trace elements zinc and copper might play an important role in the process of IVF-ET.