膝骨痹康胶囊对膝骨关节炎大鼠肿瘤坏死因子-α、转化生长因子-β1的影响

Effect of QIGUBIKANG capsules on tumor necrosis factor-alpha and transforming growth factor beta1 in the rats with knee osteoarthritis

目的:通过研究膝骨痹康胶囊对膝骨关节炎大鼠肿瘤坏死因子-α、转化生长因子-β1的影响,探讨膝骨痹康胶囊治疗膝骨关节炎的作用机制。方法:取60只健康清洁级SD大鼠,随机选取10只纳入空白对照组,其余50只采用单侧跟腱切除法制造大鼠膝骨关节炎模型,造模成功后将其随机分为模型对照组、抗骨质增生胶囊组、膝骨痹康胶囊低剂量组、膝骨痹康胶囊中剂量组、膝骨痹康胶囊高剂量组,每组10只。药物干预30 d后采用免疫组织化学法观察大鼠膝关节软骨中肿瘤坏死因子-α、转化生长因子β1的表达。结果:①肿瘤坏死因子-α。各组阳性细胞平均光密度比较,差异有统计学意义(F=9.740,P=0.000)。组间两两比较:空白对照组平均光密度小于模型对照组、膝骨痹康胶囊低剂量组和中剂量组(P=0.000;P=0.001;P=0.012),模型对照组平均光密度大于抗骨质增生胶囊组、膝骨痹康胶囊低剂量组、中剂量组和高剂量组(P=0.000;P=0.015;P=0.001;P=0.000),膝骨痹康胶囊低剂量组平均光密度大于高剂量组(P=0.024),其余各组比较,差异均无统计学意义。②转化生长因子-β1。各组阳性细胞平均光密度比较,差异有统计学意义(F=11.526,P=0.000)。组间两两比较:空白对照组平均光密度大于其余5组(P=0.000;P=0.000;P=0.001;P=0.036;P=0.000),模型对照组平均光密度小于膝骨痹康胶囊低剂量组和中剂量组(P=0.011;P=0.000),抗骨质增生胶囊组平均光密度小于膝骨痹康胶囊中剂量组(P=0.011),膝骨痹康胶囊中剂量组平均光密度大于高剂量组(P=0.007),其余各组比较,差异均无统计学意义。结论:膝骨痹康胶囊可能是通过下调膝骨关节炎患者关节软骨中肿瘤坏死因子-α的表达,同时上调转化生长因子β1的表达来发挥治疗作用的。

Objective： To explore the mechanism of action of QIGUBIKANG capsules in the treatment of knee osteoarthritis （ KOA ） through the study of its effect on tumor necrosis factor-alpha（TNF-α）and transforming growth factor betal （TGF-β1）in the rats with knee osteoarthritis. Methods：Ten cases were randomly selected from the sixty healthy SD rats of clean grade and were enrolled into the blank control group, while the others were made into KOA models with excision of unilateral achilles tendon. After the successful modeling, the 50 KOA models were randomly divided into model control group, anti-hyperostosis capsules group, low-dose-QIGUBIKANG-capsule group, medium-dose-QIGUBIKANG-capsule group and high-dose-QIGUBIKANG-capsule group, 10 cases in each group. After 30 days of drug intervention ,the expressiorts Qf TNF-α and TGF-β1 in rat knee cartilage were observed through immunohistoehemical method. Results ：①TNF- α： There was statistical difference in the average optical of positive cells among the 6 groups （ F = 9. 740, P = 0. 000 ） ; the average optical of positive cells in the blank control group was smaller than that of model control group,low-dose-QIGUBIKANG-capsule group and medium- dose-QIGUBIKANG-capsule group respectively（P = 0. 000 ;P = 0. 001 ;P = 0. 012）, and the average optical of positive cells in the model control group was higher than that of anti-hyperostosis capsules group, low-dose-QIGUBIKANG-capsule group, medium-dose-QIGUBI- KANG-capsule group and high-dose-QIGUBIKANG-capsule group respectively（ P = 0. 000 ;P = 0. 015 ;P = 0. 001 ;P = 0.000 ） ; and the average optical of positive cells in the low-dose-QIGUBIKANG-capsule group was higher than that of high-dose-QIGUBIKANG-capsule group （ P = 0. 024） ; while there was no statistical difference in the average optical of positive cells compared between any other groups. ②TGF- β1 ： There was statistical difference in the average optical of positive cells among the 6 groups（ F = 11. 526 ,P = 0.000） ; the average optical of positive cells in the blank control group was higher than that of the other 5 groups respectively （P = 0. 000;P = 0. 000;P = 0. 001 ;P = 0. 036 ;P = 0. 000）;the average optical of positive cells in model control group was smaller than that of low-dose-QIGUBIKANG-capsule group and medium-dose-QIGUBIKANG-capsule group positive cells in anti-hyperostosis capsules group was respectively （ P = 0. 011 ; P = 0. 000 ）, and the average optical of smaller than that of medium-dose-QIGUBIKANG-capsule group （P = 0. 011 ）, and the average optical of positive cells in medium-dose-QIGUBIKANG-capsule group was higher than that of high-dose-QIGUBIKANG-capsule group （ P = 0. 007 ） ; while there was no statistical difference in the average optical of positive cells compared between any other groups. Conclusion： QIGUBIKANG capsules can exert its therapeutic effects on KOA through the down-regulation of TNF-α expression in joint cartilages and the up-regulation of TGF-β1 expression.