乙型肝炎病毒对树突状细胞表型成熟和功能活化的影响

Hepatitis B virus suppress expression of costimulatory molecules and functional maturation of DCs

目的:研究乙型肝炎病毒(HBV)病毒粒子和抗原成分对树突状细胞(DC)成熟和功能活化的影响。方法:分离培养小鼠骨髓来源的DC细胞(BM-DC),在培养过程中加入含有HBV病毒粒子和抗原成分的培养基,流式细胞技术分析DC细胞表面分子的表达水平,病毒保护实验和细胞因子生物分析方法分别检测DC细胞培养上清中的干扰素(IFN)和肿瘤坏死因子(TNF)的表达水平。结果:培养基中加入HBV病毒粒子和抗原成分显著抑制DC细胞表面共刺激分子(CD40、CD80、CD86)和成熟标志分子(MHC-Ⅱ)的表达,降低DC细胞培养上清中IFN和TNF的水平。结论:HBV可以直接抑制DC的表型成熟和功能活化,有可能在HBV感染的慢性化机制中起重要作用。

Objective： To investigate the inhibitory effect of hepatitis B virus （HBV） virons and proteins on DC＇s functional maturation. Methods： Bone marrow derived DCs （BM-DC） were isolated from C57BL/6 mice, and cultured in the presence of HBV-Met cell supernatants, which contain HBV virons and proteins, the expression of surface molecules （ CD40, CD80, CD86, MHC-Ⅱ） on DCs, and IFN and TNF production in DC＇s culture supernatants were analyzed by flow cytometry, viral protection assay, and cytokines bioassay, respectively. Results： The presence of HBV virons and proteins in the DC＇s culture significantly reduce the expression of surface molecules （ CD40, CD80, CD86, MHC-Ⅱ） on DCs, and IFN and TNF production in DC＇s culture supernatants. Conclusion： HBV virons and proteins could inhibit DCs functional maturation directly, which may play an important role in the chronicity and immune tolerance of HBV infection.