双歧杆菌黏附素对大鼠肠缺血再灌注损伤的防护作用

The protective effects of bifidobacterial adhesin on ischemic reperfusion injury of intestine in rats

目的 研究双歧杆菌分泌型黏附素对大鼠缺血再灌注（I/R）后肠黏膜屏障的防护作用。方法 雄性SD大鼠72只随机分为假手术组（24只）、I/R模型组（24只）和黏附素预处理组（预处理组，24只）。建模成功后6h及1、4、7d，各组分别取6只大鼠剖杀，观察小肠组织病理改变，并检测各时间点血中TNFα、IL-6、IL-10、二胺氧化酶（DAO）和D-乳酸的活性和含量。结果 I/R模型组血中TNFα、IL-6、DAO和D-乳酸水平在各时间点均高于假手术组（P值均＜0.05），IL-10无明显差异；预处理组各时间点IL-6和DAO水平均明显低于I/R模型组（P值均＜0.05），术后1d的TNFα浓度及术后4d和7d的血浆D-乳酸浓度也低于I/R模型组（P值均＜0.05）；预处理组的小肠病理改变较I/R模型组减轻（Chiu氏评分：6 h，3.22±0.22比3.57±0.20；1d，3.77±0.13比3.90±0.12;4 d，2.93±0.23比3.07 ±0.21；7 d,2.10±0.30比2.22±0.17,P值均＜0.05）。结论 双歧杆菌黏附素对I/R后大鼠肠黏膜屏障具有防护作用，能减轻肠缺血再灌注损伤。

Objective To investigate the protection effect of bifidobacterial adhesin for intestine ischemia/reperfusion （I/R） injury on gut barrier function in rat.Methods Seventy-two male SD rats were randomly divided into sham operation group （n =24）, I/R model group （n =24） and pretreatment group of bifidobacterial adhesin （pretreatment group, n = 24）.Six rats were anatomized at 6 h, 1 d, 4 d and 7d after inducing I/R model in each group, respectively.The pathological changes of the terminal ilea and the blood levels of TNFα, IL-6, IL-10, diamine oxidase （DAO）, and the activity and content of D-lactic acid were observed.Results The blood levels of TNFα, IL-6, DAO and D-lactic acid in I/R model group were significantly higher than sham operation group at all time points （P 〈0.05） , while the blood level of IL-10 was no significantly change.The activity of IL-6 and DAO in pretreatment group was significantly lower than I/R model group at all time points （P 〈 0.05）, the blood level of TNFαt in pretreatment group was significantly lower than I/R model group at 1 d, the blood level of D-lactic was significantly lower than I/R model group at 4 d and 7 d （P 〈 0.05）. Intestinal pathological damages were obviously milder in pretreatment group than I/R model group at all time points （Chiu＇s pathological scores： 6 h, 3.22 ±0.22 vs 3.57 ±0.20;1 d,3.77 ±0.13 vs 3.90 ±0.12;4 d,2.93 ±0.23 vs 3.07 ±0.21;7 d,2.10 ±0.30 vs 2.22 ±0.17,all P 〈 0.05）.Conclusion The pretreatment of bifidobacterial adhesin could protect the intestinal mucosa from I/R injury, and alleviate intestinal ischemic reperfusion injury.