复方尿囊素片对乙醇所致大鼠胃黏膜损伤保护作用的实验研究

Protective effects of aldioxa tablets on ethanol-induced gastric mucosal lesions in rats

目的研究复方尿囊素片对乙醇所致大鼠急性胃黏膜损伤的预防保护作用及其机制。方法 60只健康雄性Wistar大鼠随机分为5组:乙醇损伤组、空白对照组、硫糖铝保护组、氢氧化铝保护组、复方尿囊素保护组。保护组分别用硫糖铝、氢氧化铝及复方尿囊素片提前给大鼠灌胃,其后用乙醇灌胃致急性胃黏膜损伤,然后分别测定各组胃黏膜溃疡指数、黏膜损伤积分、胃黏膜血流、黏膜前列腺素E2(PGE2)及一氧化氮(NO)含量,并在显微镜及电镜下观察胃黏膜组织学改变。结果复方尿囊素保护组的各项检测指标显示:溃疡指数、损伤积分显著低于乙醇损伤组(P=0.000,P=0.000),明显低于氢氧化铝保护组(P=0.020,P=0.004),也低于硫糖铝保护组,但差异无统计学意义(P=1.000,P=1.000);黏膜血流值较乙醇损伤组明显增加(P=0.000),明显高于氢氧化铝保护组(P=0.019),与硫糖铝保护组的差异无统计学意义(P=0.266);黏膜PGE2及NO含量均较乙醇损伤组明显增加(P=0.000,P=0.001),明显高于氢氧化铝保护组(P=0.002,P=0.001),也高于硫糖铝保护组,但差异无统计学意义(P=0.451,P=0.199)。结论复方尿囊素片对乙醇所致大鼠急性胃黏膜损伤有明显的预防保护作用,其作用是通过增加胃黏膜血流、增加黏膜PGE2及NO含量等机制实现的。

Objective To study the protective effects of aldioxa tablets on ethanol-induced gastric mucosal lesions in rats,and explore the potential pathogenesis.Methods Sixty healthy male Wistar rats were randomly divided into five groups： injury group,control group,sucralfate protective group,aluminium hydroxide protective group and aldioxa protective group.The three protective groups were treated with sucralfate,aluminium hydroxide and aldioxa tablets respectively before to induce gastric mucosal injury.Then the ulcer index,epithelial damage scoring,blood flow of gastric mucosa,the concentration of prostaglandin E2 and nitric oxide of gastric mucosa were measured,and the pathological changes on histological sections and ultrastructural sections of gastric mucosa were assessed under microscope or electron microscope.Results Compared with injury group,the ulcer index and epithelial damage scoring of gastric mucosa significantly decreased in aldioxa protective group（P=0.000,P=0.000）,the blood flow of gastric mucosa significantly increased in aldioxa protective group（P=0.000）,prostaglandin E2 and nitric oxide concentration of gastric mucosa significantly increased in aldioxa protective group（P=0.000,P=0.001）.Conclusion The data revealed that aldioxa tablets had a significant protection effect on rats with acute gastric mucosa injury induced by ethanol,and the increase in blood flow,prostaglandin E2 and nitric oxide concentration maybe contribute to the protective effects.